温度和盐度对刺枝鱼栖苔(Acanthophora spicifera)(红藻门, 松节藻科)生长及其几种光合色素的影响   总被引：4，自引：3，他引：1  

丁兰平  孙国栋  黄冰心  陈善文  陈伟洲 《海洋与湖沼》2013,44(4):913-918

通过正交试验研究了温度和盐度对刺枝鱼栖苔(Acanthophora spicifera)生长及其光合色素的影响, 以探明刺枝鱼栖苔生长的最适温度及盐度条件。结果表明, 刺枝鱼栖苔生长的适宜温度范围为20—30℃, 最适温度为25℃, 在此温度条件下其相对生长率(RGR)最大为4.33%; 适宜盐度范围为15—35, 最适盐度范围为20—25, 随盐度的继续升高, 刺枝鱼栖苔RGR呈下降趋势。温度和盐度对刺枝鱼栖苔光合色素(chl a、Car、PE和PC)的影响显著, 高盐胁迫能明显降低刺枝鱼栖苔光合色素的含量, 而高温能促进其藻胆蛋白的合成与积累。  相似文献   

江蓠属藻胆体的研究 Ⅱ.藻胆体的荧光光谱特性     

张学成  程晓杰  隋正红  魏东  刘晨临  徐年军 《中国海洋大学学报(自然科学版)》1999,(3)

报道以青岛野生型龙须菜（ｗｔ）、黄色突变体（ｙｅ１００）、４株绿色突变体（ｇｒ１８０、ｇｒ１８４、ｇｒ１８６、ｇｒ１８８）、委内瑞拉野生型龙须菜（ｌｖ）、南非野生型龙须菜（ｓａ）及真江蓠（ｇａ）为材料,用蔗糖密度梯度离心法得到完整的藻胆体。用５４０ｎｍ激发,野生型的荧光发射峰位于５７８ｎｍ和６７０ｎｍ,相对荧光强度ＦＡＰＢ／ＦＰＥ的比值为１．５０。几种材料的ＰＥ和ＡＰＢ荧光发射峰没有明显区别（不超过２ｎｍ）,但两峰荧光强度比值显著差异。结果显示ｌｖ的ＦＡＰＢ／ＦＰＥ比值最低,只有１．２０,ｇｒ１８６的比值最高,为５．６７,野生型中ｓａ也比较高,为４．７９。用６７０ｎｍ激发,野生型龙须菜的荧光社发光谱有５个峰,分别为４９９ｎｍ、５４０ｎｍ、５６８ｎｍ、６１４ｎｍ、６５０ｎｍ,与其吸收光谱一一对应。野生型和突变体、不同产地龙须菜及真江蓠的荧光发射峰的波长都与其吸收光谱相对应。  相似文献   

Effects of waterlogging,salinity and light on the productivity of Bruguiera gymnorrhiza and Heritiera littoralis seedlings     

MM Mangora  MSP Mtolera  M Björk 《African Journal of Marine Science》2017,39(2):167-174

This study aimed to establish the effects of waterlogging, salinity and light on the early development of mangroves. Seedlings of Bruguiera gymnorrhiza (L.) Lamk. and Heritiera littoralis Dryand were exposed to 12 weeks of waterlogging, during which time growth and photosynthesis were measured every two weeks. The salinity of the water inundation ranged from fresh water to full-strength sea water (salinity 35). Seedlings were exposed to either full sunlight of 1 500 µmol photon m^–2 s^–1 (SD 397) at midday or shade conditions of 325 µmol photon m^–2 s^–1 (SD 40) of light at midday, to explore whether the plants would be differently affected by prolonged waterlogging in increased salinities and under different light conditions. Heritiera littoralis was more sensitive to waterlogging, salinity and light, displaying a least relative growth rate of 0.127 g g^–1 week^–1 (SE 0.032) under shade, and 0.025 g g^–1 week^–1 (SE 0.021) in full light; while under shade, photosynthesis continued only in fresh water, but photosynthetic yield decreased from 0.7 to 0.4 with increasing duration of waterlogging. By 12 weeks, all H. littoralis seedlings treated with any saltwater mixture had died. Bruguiera gymnorrhiza seedlings maintained a moderate rate of photosynthesis throughout inundation in both shade and full light, with yields of 0.7 and 0.3, respectively. Furthermore, B. gymnorrhiza survived waterlogging in up to 66% seawater, and maintained comparable relative growth rates of 0.164 g g^–1 week^–1 (SE 0.066) with 0.083 g g^–1 week^–1 (SE 0.065) and 0.074 g g^–1 week^–1 (SE 0.036) with 0.052 g g^–1 week^–1 (SE 0.037) under shade and in full light between fresh water and the highest salinity conditions, respectively. These results suggest that B. gymnorrhiza is broadly tolerant, making it a potential candidate species for restoring vulnerable mangrove forests.  相似文献   

基于光谱吸收特征分析的彩绘文物颜料识别研究     

张陈峰  胡云岗  侯妙乐  吕书强  张学东 《地理信息世界》2017,24(3)

高光谱成像技术作为一种无损高效的检测方法,对于彩绘文物颜料的鉴别具有重要意义。由于全波段参与光谱相似度计算会造成数据的冗余,没有充分利用光谱的细微特征。为此,本文首先对文物颜料光谱吸收特征进行参量化分析,并通过改进的光谱吸收特征拟合算法与标准光谱进行匹配识别,从而得到识别结果。实验以获取的一幅波长为400~1000nm的古代壁画高光谱影像为例,通过光谱吸收特征分析识别出壁画的颜料主要成分有朱砂、赭粉、石绿和石青,4种颜料的光谱吸收特征拟合度分别是:0.95、0.77、0.92、0.81。实验结果表明:对光谱吸收特征分析可以帮助识别彩绘文物的颜料信息,该方法可为以后文物修复提供参考。  相似文献   

Influence of non-photosynthetic pigments on light absorption and quantum yield of photosynthesis in the western equatorial Pacific and the Subarctic North Pacific     

Anil Kumar Vijayan  Takashi Yoshikawa  Shigeki Watanabe  Hiroaki Sasaki  Kazuhiko Matsumoto  Sei-Ichi Saito  Shigenobu Takeda  Ken Furuya 《Journal of Oceanography》2009,65(2):245-258

Regional variations in the contribution of non-photosynthetic pigments (ā _np^*) to the total light absorption of phytoplankton (ā _ph^*) and its influence on the maximum quantum yield of photosynthesis (φ _m) were investigated. In the western equatorial Pacific, the surface ā _np^* : ā _ph^* ratio was higher in the western warm pool than that in the upwelling region. This difference appears to be attributable to severe nitrate depletion and higher percentage of prokaryotes, which can accumulate very high concentrations of zeaxanthin in the western warm pool. In the subarctic North Pacific, the ā _np^* : ā _ph^* ratio was expected to be higher in the Alaskan Gyre where the thermocline is sharper and iron limitation may possibly be more severe than in the Western Subarctic Gyre. However, the ratio was actually higher in the Western Subarctic Gyre, contradictory to our expectations. This east-west variation appears to be attributable to changes in the taxonomic composition; cyanobacteria were more abundant in the Western Subarctic Gyre. The values of ā _np^* : ā _ph^* and its vertical variations were relatively small in the subarctic North Pacific compared to those in the western equatorial Pacific. These inter-regional variations appear to be attributable to the lower solar radiation intensity, smaller percentage of cyanobacteria, and relatively strong vertical mixing in the subarctic North Pacific. The spatial variations in ā _np^* : ā _ph^* significantly influence φ _m. In comparison with φ _m based on the total light absorption (φ _{m ph}), the values corrected for the contribution of non-photosynthetic pigments (φ _{m ps}) showed an increase in both the western equatorial Pacific and the subarctic North Pacific.  相似文献   

The absorption of water color components and spectral modes in the Pearl River estuary     

席红艳  丘仲锋  何宜军  简伟军 《中国海洋湖沼学报》2007,25(4):359-366

In-situ data from cruises in the Pearl River estuary and adjacent marine areas were collected during March to May 2001. The absorption coefficients of the water color components were studied in detail containing total suspended matter (TSM), chlorophyll-a (chl-a), colored dissolved organic matter (CDOM), and de-pigment particles. For absorption coefficient of TSM, ap, and that of de-pigment particles, ad, correlations of ap(440)-TSM, ad(440)-TSM, ap(440)-chl-a and ad-chl-a were done (the italicized term means the concentration). There was a good correlation between ap(440) and chl-a concentration. An empirical relationship model between aph(675) and chl-a was developed showing a strong correlation of 0.93. Based on the two models the chl-a and aph(λ) were correlated. The values of calculated empirical spectral slope for CDOM absorption coefficients and that of de-pigment particles, 0.017 0 and 0.011 6 respectively, both are within a relative standard error of 10.0%.  相似文献   

Effects of heavy metals (Pb2+ and Cd2+) on the ultrastructure, growth and pigment contents of the unicellular cyanobacterium Synechocystis sp. PCC 6803     

K. K. I. U. Arunakumara  ZHANG Xuecheng 《中国海洋湖沼学报》2009,27(2)

The unicellular cyanobacterium Synechocystis sp. PCC 6803, a model organism known for its unique combination of highly desirable molecular genetic, physiological and morphological characteristics, was employed in the present study. The species was cultured in BG11 liquid medium contained various initial concentrations of Pb2+ and Cd2+ (0, 0.5, 1, 2, 4, 6 and 8 mg/L). The experiment was conducted for six days and the metal induced alterations in the ultrastructure, growth and pigment contents were assessed. Alterations in the ultrastructure of the Synechocystis sp. PCC 6803 ceils became evident with the increased (>4 mg/L Pb2+) metal concentration. The photosynthetic apparatus (thylakoid membranes) were found to be the worst affected. Deteriorated or completely destroyed thylakoid membranes have made large empty spaces in the cell interior. In addition, at the highest concentration (8 mg/L pb2+), the polyphosphate granules became more prominent both in size and number. Despite the initial slight stimulations (0.2, 3.8 and 6.5% respectively at 0.5, 1 and 2 mg/L Pb2+), both metals inhibited the growth in a dose-dependent manner as incubation progressed. Pigment contents (chlorophyll a, βcarotene and phycocyanin) were also decreased with increasing metal concentration. Cells exposed to 6 mg/L Pb2+, resulted in 36.56, 37.39 and 29.34% reductions of chlorophyll a, β carotene and phycocyanin respectively over the control. Corresponding reductions for the same Cd2+concentrations were 57.83, 48.94 and 56.90%. Lethal concentration (96 h LC50) values (3.47 mg/L Cd2+ and 12.11 mg/L Pb2+) indicated that Synechocystis sp. PCC 6803 is more vulnerable to Cd2+ than Pb2+.  相似文献   

线性/非线性光谱混合模型估算白刺灌丛植被覆盖度     

姬翠翠  贾永红  李晓松  王金英 《遥感学报》2016,20(6):1402-1412

及时监测干旱与半干旱区光合/非光合植被覆盖度时空变化,可以为指导荒漠化防治工程及植被衰退机制研究提供重要信息。本文以甘肃民勤典型植被白刺灌丛为研究对象,通过地面控制性光谱实验获取混合光谱、端元光谱与丰度信息,开展线性与非线性光谱混合模型(包括核函数非线性和双线性混合模型)估算光合和非光合植被覆盖度的对比研究,采用全限制最小二乘法进行模型解混,分别获取各样本数据中各类端元丰度及其精度信息,通过模型分解的均方根误差(RMSE)与地面验证精度确定用于光合和非光合植被覆盖度估算的最佳光谱混合模型,其中参考端元丰度采用神经网络(NNC)分类算法对数字影像进行分类获取。结果表明:(1)引入阴影端元的四端元模型相对于传统的三端元模型(光合/非光合植被与裸土)能有效提高光谱解混的精度,并提高光合和非光合植被覆盖度估算精度;(2)对白刺灌丛来说,光合植被、非光合植被、裸土及阴影间多重散射混合效应存在,但混合效应不够显著;考虑非线性参数的核函数非线性光谱混合模型表现略低于线性光谱混合模型,因此非线性光谱混合模型在估算白刺灌丛光合和非光合植被覆盖度时相对于线性光谱混合模型没有明显优势;(3)基于光合/非光合植被、裸土与阴影四端元的线性光谱混合模型可以实现白刺灌丛光合和非光合植被覆盖度的准确估算,光合植被覆盖度估算RMSE为0.11 77,非光合植被覆盖度估算RMSE为0.0835。  相似文献   

长江口邻近海域浮游细菌分布与环境因子的关系   总被引：5，自引：0，他引：5  

李云  李道季 《海洋通报》2007,26(6):9-18

利用2003年9-10月"东海陆架水交换"调查航次期间获得的浮游异养细菌、寡营养细菌和光合细菌的分布资料和实测水环境数据,探讨了这三类细菌的分布特征及其与相关环境因子的关系。结果表明:在长江口邻近海域,以30等盐线为界,三类细菌的空间分布具有显著差异;调节制约这三类细菌类群生长和分布的环境因子不同;表层硝酸盐浓度1μmol/L可作为一个特征浓度,高于1μmol/L的海域,陆源输入的硝酸盐对于这三类细菌的生长和分布具有重要影响,低于1μmol/L的海域,硝酸盐的影响较小或无影响。同时,采用法国梅里埃API STAPH系统,结合电镜观察,对两株已获得纯培养的有色菌株进行了属种的鉴定,分别是变异微球菌(Micrococcus varians)和玫瑰微球菌(Micrococcus roseus),目前尚未发现这两种细菌在长江口及其邻近海域分布的报道。  相似文献   

Grazing impact of mesozooplankton in an upwelling region off northern California, 2000–2003     

Anne M. Slaughter  Stephen M. Bollens  Gretchen Rollwagen Bollens   《Deep Sea Research Part II: Topical Studies in Oceanography》2006,53(25-26):3099

Mesozooplankton (>200 μm) grazing impact (% phytoplankton standing crop consumed d⁻¹) was determined by the gut fluorescence method during three springs (2000, 2001 and 2002) and two winters (2002 and 2003) in a coastal upwelling region off northern California. Wind events, in terms of both magnitude and duration, varied inter-annually and seasonally and included both upwelling-favorable and relaxation events. Grazing impact of mesozooplankton also varied inter-annually and seasonally, and was highest during June 2000 (mean=129% of standing crop d⁻¹), a prolonged period of wind “relaxation” and phytoplankton bloom. In contrast, mean grazing impact was lower during periods of stronger, more persistent winds, more active upwelling, greater cross-shelf transport, and lower chlorophyll concentration (25% and 38% in May–June 2001 and 2002, respectively). Wintertime conditions (January 2002 and 2003) were characterized by weakly upwelling or downwelling-favorable winds, low chlorophyll concentration, and lower mean mesozooplankton grazing impact (13% and 12%, respectively). The larger (>500 μm) size class contributed proportionally more to total mesozooplankton (>200 μm) grazing impact than the smaller (200–500 μm) size class during all sampling periods except spring 2002. These results suggest that mesozooplankton grazing impact is higher in spring than in winter, and that during the spring upwelling season, grazing is higher during periods of wind relaxation (weak upwelling) than during periods of stronger upwelling. Further, these results suggest an important role of mesozooplankton grazers on phytoplankton dynamics in the upwelling region off northern California.  相似文献